PCR Detection of an Eye Anomaly in a Family of Longhaired Collies

Abstract Inherited eye diseases have been the subject of genetic research for many years. This paper focuses on optimisation DNA test based polymerase chain reaction (PCR) detection Collie Eye Anomaly (CEA) in dogs. A small family four longhaired Collies (parents and their daughters) with a confirmed positive clinical ophthalmologic examination CEA served as source affected animals. Both PCR conditions examined were suitable detecting canine NHEJ1 gene mutation associated CEA. One carrier was found group eleven randomly selected control healthy The previous CEA-positive ophthalmological Collies. results indicated that all members had homozygous intronic deletion 7799 bases gene. female may potentially transmit this CEA-associated to puppies.